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nebnext ultra ii ligation module  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext ultra ii ligation module
    Nebnext Ultra Ii Ligation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+ultra+ligation+module/NEBNext+Ultra+II+Ligation+Module/pmc13171454-68-9-15
    Average 97 stars, based on 519 article reviews
    nebnext ultra ii ligation module - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    other:

    Article Title: ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13.
    Article Snippet: After reverse crosslinking, ChIP and input DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (E7442; NEB) followed by adaptor ligation with the NEBNext Ultra Ligation Module (E7445; NEB).

    Article Title: ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13
    Article Snippet: After reverse crosslinking, ChIP and input DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (E7442; NEB) followed by adaptor ligation with the NEBNext Ultra Ligation Module (E7445; NEB).

    Chromatin Immunoprecipitation:

    Article Title: NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis
    Article Snippet: .. After washing, elution and reverse crosslinking, the ChIP DNA was purified and used to prepare ChIP–seq libraries by using NEBNext End Repair/dA-Tailing Module (NEB, cat. no. E7442) followed by adaptor ligation with NEBNext Ultra Ligation Module (NEB, cat. no. 4775). .. The ChIP and input DNA libraries were amplified for 15 cycles and sequenced using the Illumina NovaSeq 6000 platform with paired-end 1×75 as the sequencing mode.

    Article Title: Beclin 1 prevents ISG15-mediated cytokine storms to secure fetal hematopoiesis and survival
    Article Snippet: .. After reverse cross-linking, ChIP and input DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (E7442, New England Biolabs) followed by adaptor ligation with the NEBNext Ultra Ligation Module (E7445, New England Biolabs). .. The DNA libraries were amplified and sequenced using an Illumina NovaSeq 6000.

    Article Title: NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis.
    Article Snippet: .. After washing, elution and reverse crosslinking, the ChIP DNA was purified and used to prepare ChIP–seq libraries by using NEBNext End Repair/dA-Tailing Module (NEB, cat. no. E7442) followed by adaptor ligation with NEBNext Ultra Ligation Module (NEB, cat. no. 4775). .. The ChIP and input DNA libraries were amplified for 15 cycles and sequenced using the Illumina NovaSeq 6000 platform with paired-end 1×75 as the sequencing mode.

    Purification:

    Article Title: NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis
    Article Snippet: .. After washing, elution and reverse crosslinking, the ChIP DNA was purified and used to prepare ChIP–seq libraries by using NEBNext End Repair/dA-Tailing Module (NEB, cat. no. E7442) followed by adaptor ligation with NEBNext Ultra Ligation Module (NEB, cat. no. 4775). .. The ChIP and input DNA libraries were amplified for 15 cycles and sequenced using the Illumina NovaSeq 6000 platform with paired-end 1×75 as the sequencing mode.

    Article Title: NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis.
    Article Snippet: .. After washing, elution and reverse crosslinking, the ChIP DNA was purified and used to prepare ChIP–seq libraries by using NEBNext End Repair/dA-Tailing Module (NEB, cat. no. E7442) followed by adaptor ligation with NEBNext Ultra Ligation Module (NEB, cat. no. 4775). .. The ChIP and input DNA libraries were amplified for 15 cycles and sequenced using the Illumina NovaSeq 6000 platform with paired-end 1×75 as the sequencing mode.

    Ligation:

    Article Title: NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis
    Article Snippet: .. After washing, elution and reverse crosslinking, the ChIP DNA was purified and used to prepare ChIP–seq libraries by using NEBNext End Repair/dA-Tailing Module (NEB, cat. no. E7442) followed by adaptor ligation with NEBNext Ultra Ligation Module (NEB, cat. no. 4775). .. The ChIP and input DNA libraries were amplified for 15 cycles and sequenced using the Illumina NovaSeq 6000 platform with paired-end 1×75 as the sequencing mode.

    Article Title: DNA construct for sequencing and method for preparing the same
    Article Snippet: End Repair/dA-Tailing Follow the NEBNext® UltraTM End Repair/dA-Tailing Module (NEB #E7442) protocol: Mix the following components in a sterile, nuclease-free tube: (green) End Prep Enzyme Mix—3.0 μl; (green) End Repair Reaction Buffer (10×)—6.5 μl; PCR amplicons from previous step—55.5 μl; Mix by pipetting, followed by a quick spin to collect all liquid from the sides of the tube. .. Place in a thermocycler, with the heated lid on, and run the following program: 30 minutes @ 20° C.; 30 minutes @ 65° C.; Hold at 4° C. Proceed directly to NEBNext Ultra Ligation Module (NEB #E7445): If DNA input prior to End Repair is <100 ng, dilute the xGen® Dual Index UMI Adapters 1:10 in 10 mM Tris-HCl pH 7.5-8.0 or 10 mM Tris-HCl pH 7.5-8.0 with 10 mM NaCl to a final concentration of 1.5 μM. ..

    Article Title: Beclin 1 prevents ISG15-mediated cytokine storms to secure fetal hematopoiesis and survival
    Article Snippet: .. After reverse cross-linking, ChIP and input DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (E7442, New England Biolabs) followed by adaptor ligation with the NEBNext Ultra Ligation Module (E7445, New England Biolabs). .. The DNA libraries were amplified and sequenced using an Illumina NovaSeq 6000.

    Article Title: Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation
    Article Snippet: The fragmented DNA was converted to end-repaired, adenylated DNA using NEBNext Ultra End Repair/dA-Tailing Module (NEB #7442L). .. Methylated adaptors (NEBNext Multiplex Oligos for Illumina; NEB #E7535L) were ligated to the product from the preceding step using NEBNext Ultra Ligation Module (NEB #7445L). .. The resulting product was size-selected as described in the manufacturer’s protocol by employing modified SPRIselect (Beckman Coulter #B23318) bead ratios of 0.37X and 0.2X to select for an insert size of 300–400 bp.

    Article Title: NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis.
    Article Snippet: .. After washing, elution and reverse crosslinking, the ChIP DNA was purified and used to prepare ChIP–seq libraries by using NEBNext End Repair/dA-Tailing Module (NEB, cat. no. E7442) followed by adaptor ligation with NEBNext Ultra Ligation Module (NEB, cat. no. 4775). .. The ChIP and input DNA libraries were amplified for 15 cycles and sequenced using the Illumina NovaSeq 6000 platform with paired-end 1×75 as the sequencing mode.

    Article Title: DNA construct for sequencing and method for preparing the same
    Article Snippet: NEBNext® UltraTM End Repair/dA-Tailing Module (NEB #E7442). .. NEBNext® Ultra Ligation Module (NEB #E7445). ..

    Concentration Assay:

    Article Title: DNA construct for sequencing and method for preparing the same
    Article Snippet: End Repair/dA-Tailing Follow the NEBNext® UltraTM End Repair/dA-Tailing Module (NEB #E7442) protocol: Mix the following components in a sterile, nuclease-free tube: (green) End Prep Enzyme Mix—3.0 μl; (green) End Repair Reaction Buffer (10×)—6.5 μl; PCR amplicons from previous step—55.5 μl; Mix by pipetting, followed by a quick spin to collect all liquid from the sides of the tube. .. Place in a thermocycler, with the heated lid on, and run the following program: 30 minutes @ 20° C.; 30 minutes @ 65° C.; Hold at 4° C. Proceed directly to NEBNext Ultra Ligation Module (NEB #E7445): If DNA input prior to End Repair is <100 ng, dilute the xGen® Dual Index UMI Adapters 1:10 in 10 mM Tris-HCl pH 7.5-8.0 or 10 mM Tris-HCl pH 7.5-8.0 with 10 mM NaCl to a final concentration of 1.5 μM. ..

    Methylation:

    Article Title: Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation
    Article Snippet: The fragmented DNA was converted to end-repaired, adenylated DNA using NEBNext Ultra End Repair/dA-Tailing Module (NEB #7442L). .. Methylated adaptors (NEBNext Multiplex Oligos for Illumina; NEB #E7535L) were ligated to the product from the preceding step using NEBNext Ultra Ligation Module (NEB #7445L). .. The resulting product was size-selected as described in the manufacturer’s protocol by employing modified SPRIselect (Beckman Coulter #B23318) bead ratios of 0.37X and 0.2X to select for an insert size of 300–400 bp.

    Multiplex Assay:

    Article Title: Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation
    Article Snippet: The fragmented DNA was converted to end-repaired, adenylated DNA using NEBNext Ultra End Repair/dA-Tailing Module (NEB #7442L). .. Methylated adaptors (NEBNext Multiplex Oligos for Illumina; NEB #E7535L) were ligated to the product from the preceding step using NEBNext Ultra Ligation Module (NEB #7445L). .. The resulting product was size-selected as described in the manufacturer’s protocol by employing modified SPRIselect (Beckman Coulter #B23318) bead ratios of 0.37X and 0.2X to select for an insert size of 300–400 bp.



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